The topic was on microbial growth. Basically for the first part, it describes the physical and chemical requirements for microbial growth.
Physical : light, temperature, pH, water activity and osmotic pressure.
Chemical : electron donor, electron acceptor, micronutrients ( vitamins, amino acids, trace minerals)
Next, it was about isolation/culturing, maintaining and preserving microbes.
The steps in aseptic technique are:
1. Flame all caps and lids
2. Tightly close all caps
3. Remove materials from the hood
4. Turn off gas
5. Wash the hood surface
6. Turn the UV light on to disinfect
Pure culture is a population of identical originating from a single cell.
Sub culture: the process of transferring bacteria from grown culture to new culture (fresh agar).
Methods obtaining pure culture: Streak plate method, pour plate method.
Special culture techniques: Culture anaerobic organisms.
And the topic was continue on Thursday.
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