Basically we are still in our second topic which is MICROSCOPY.
We proceed to staining. There are smears-step involved, which are preparing smears, fixation and staining.
Under fixation, there are : heat fixing
: chemical fixing
Importance of fixation is to preserves internal & external structures and fixed them in position.
3 types of staining : Simple staining
: Differential staining
: Special staining
Dyes or stains give colour so it will be more visible under microscope and easier to differentiate. Differential staining includes gram staining and acid-fast staining.
Difference between simple staining and gram staining is simple staining uses single staining while gram staining uses 2 stains, crystal violet & safranin.
Procedure involved in gram staining are fixation (crysal violet), iodine treatment, decolourization, and counter stain (safranin).
Gram positive(usually rod-shape) maintain in purple colour as it wont decolourized. Gram negative (usually coccus) - pink.
Acid fast-staining is used instead of other staining because it involves fastidious bacteria.
Eg : Mycobacterium
Special staining will only stain specific structures which are endospore, flagella and negative staining.
That's all for this topic. and we did proceed to third topic, PROKARYOTES.
We were asked to make a mind map using application such as examtime (I only remember this as my group used this for our mind map)
This is only for the external, and we are going to make mind map on the internal parts individually.
Never knew about all these applications before huhuhu. If Dr Wan did not told us about it, surely I will still use microsoft words to make a mind map haha.
That's all :)
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